10. ways of handling an autoclave
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10. ways of handling an autoclave
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—Wearing appropriate Personal Protective Equipment (PPE) including a lab coat, heat resistant gloves, and eye protection, especially when unloading the autoclave.
—Never sealing containers; under pressure they pose an explosion risk.
—Never opening the door to the autoclave if there is water running out the bottom. Clogged steam lines, equipment malfunction, or plugged drains may cause a buildup of scalding water.
—Waiting for the pressure to reach zero and the temperature is at or below 121°C before opening the door at the end of a cycle to avoid steam burns and shattered glassware. Do not stand directly in front of the door.
—Never superheating liquids. Superheating is a condition that occurs when liquids are at a temperature above their normal boiling point but do not appear to be boiling. Any disturbance of the liquid could cause some of it to violently flash to steam and spraying.
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